The fermentation of mannitol by salmonellae is said to correct the alkaline ph swing which can occur during incubation. Withdraw approximately 1ml of the supernatant and inoculate 10 ml of selenite broth. Selenite broth is recommended as enrichment media for the isolation of salmonellae from faeces, urine or other pathological materials. Solid medium contains agar at a concentration of 1. Selenite broth was developed by leifson for enrichment of salmonella from sources such as feces, urine, and food. Casein enzymic hydrolysate provides nitrogenous substances and other essential ingredients. Selenite lactose broth is used for enrichment culture of salmonellae from feces, food products, water and other materials. Selenite broth selenitef broth is used as an enrichment medium buffered with lactose peptone broth to which sodium biselenite is added as the selective agent for the isolation of salmonella from feces, urine, water, foods and other materials of sanitary importance. The pentahydrate na 2 seo 3 h 2 o 5 is the most common watersoluble selenium compound. Remove the need for pipetting, dispensing or autoclaving with convenient, prefilled and readytouse tubes of selenite broth. Not all products are available for sale in all territories. Remel selenite cystine broth thermo fisher scientific. Selenitef broth is an enrichment medium for the isolation of salmonella and some species of shigella.
F in selenite f broth is faeces, because it is mostly used for the isolation of salmonella and shigella from faecal specimens. Pi7283, rev 05, march 2008 selenite cystine broth 7283 intended use selenite cystine broth is used for the selective enrichment of salmonella species. It contains watersoluble substances of animal tissue, which include carbohydrates, organic nitrogen compounds, water soluble vitamins, and. Composition of selenite f broth casein enzymic hydrolysate 5 gml lactose 4 gml sodium phosphate 10 gml sodium hydrogen selenite nahseo3 4. A seleniumcontaining anion or ionic compound with the seo 3 2. Selenite cystine broth is a modifica tion north and bartram, 1953 of the original leifson 1936 formula, differing only in the addition of lcystine which is considered to enhance salmonella growth by reduction of toxicity. Selenite f broth culture media online microbiology notes. Based on buffered mannitol broth, it is made selective by the addition of sodium biselenite.
It is the most widely used enrichment medium to isolate salmonella typhi from fecal specimen of suspected patients andor from carriers during salmonella outbreaks. Summary and explanation selenite broth was devised by leifson,1 who demonstrated that. Summary and explanation leifson found that selenite inhibited fecal streptococci and coliforms during the first 812 hours of incubation, thereby. Selenite f broth is widely used as an enrichment medium for the. It is a buffered lactose peptone broth to which sodium biselenite is added as the selective agent. Selenite broth base manufacturer trade name selenite broth base product no. Selenite mannitol broth is an alternative to selenite f broth for the selective enrichment of salmonellae spp from clinical, food and environmental specimens. Selenite f broth composition, principle, uses, preparation and result interpretation. Can we use selenite f broth media to revive organisms like. Remel and oxoid products are now part of the thermo scientific brand, combining powerful manual, semiautomated and fully automated test. Shop online for a wide selection of bd bbl mycoflask selenitef broth prepared media for isolation of salmonella from feces, urine, water, foods and other materials of sanitary. Enzymatic digest of casein and animal tissue supply nitrogenous substances and carbohydrates for bacterial growth.
Remel selenite cystine broth is selective enrichment broth for isolation of salmonella spp. They recommended the use of this principle for the examination of sewage and river water containing large numbers of. Silver selenite, an inorganic compound of formula ag 2 seo 3. If possible even larger samples are preferable with proportionately larger amounts of the enrichment broths being used. Sodium selenite is the inorganic compound with the formula na 2 seo 3. Recommended as enrichment media for the isolation of salmonellae from faeces, urine or other pathological materials. Beef extract is an aqueous extract of lean beef tissues. Reduce media preparation time and simultaneously enrich and isolate salmonella spp. The efficiency of strontium selenite and selenite f broths in the isolation of s. Selenite cystine broth is an internationally accepted medium see icmsf, 1978.
This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements. Selenite broth is used as a selective enrichment for the cultivation of salmonella spp. Selenitef broth was devised by leifson, who demonstrated that selenite was inhibitory for coliforms and certain other microbial species, such as fecal streptococci, present in. Selenite f broth twin pack m025s recommended is used as an enrichment medium for isolation of shigella from food samples. Criterion selenite cystine broth for the detection of. Technical information selenite broth selenite f broth twin pack product code. It used as a source of selenium, especially for patients. Selenite f broth eb02e typical formula grams per litre peptone 5. Selenite f broth is recommended as an enrichment medium for the isolation of salmonella species from faeces, urine or other pathological materials in accordance with indian pharmacopoeia. Section 1 identification of the substancemixture and of. Nutrient broth contains same ingredients except agar. Page 1 of 7 sds 7155 rev 0 june 2016 section 1 identification of the substancemixture and of the company 1. Pancreatic digest of casein 5gl, lactose 4gl, sodium selenite 4gl, sodium phosphate 10gl. New selenite selective enrichment medium for the isolation of typhoid and paratyphoid bacilli.
Chemtrec usa 8004249300 safety data sheet r454582 supplier oxoid limited wade road basingstoke. Mccance, in laboratory methods in microbiology, 1966. Sodium hydrogen selenite or detecting faecal coliforms drinking in water waste water, seawater and foods samples by mpn method. Selenite cystine broth for microbiology sigmaaldrich. Sodium selenite bioreagent, suitable for cell culture catalog number s5261 storage at room temperature cas rn 10102188 synonym. Rv medium made from its individual ingredients and incubated at 42 degrees c was compared with a commercial preparation of sc broth. Bacterial culture media can be classified on the basis of composition, consistency, and purpose classification of bacterial culture media on the basis of consistency solid medium. Selenite broth is used as a selective medium for the isolation of salmonella species. Characteristics of the components used in nutrient agarbroth.
Summary and explanation selenite broth was devised by leifson,1 who demonstrated that selenite was inhibitory for coliforms and certain other microbial. At the same time, the number of false positive isolates accruing to strontium selenite broth. Selenite broth selenite f broth twin pack m052 intended use. The relative effectiveness of rappaportvassiliadis rv medium, selenite cystine sc broth, and tetrathionate tt broth for the recovery of salmonella spp. Selenite broth is used as a selective enrichment for the cultivation of. Dulcitol selenite broth selenitef broth w dulcitol. Selenite broth selenite f broth is used as an enrichment medium for the isolation of salmonella from feces, urine, water, foods and other materials of sanitary importance. Further study of strontium selenite and selenite f broths. A selenium compound with the molecular formula h2so3. A rise in ph decreases selective activity of selenite. Product summary and explanation the growth and isolation of salmonella in food and other samples can be suppressed from foodprocessing procedures including exposure to low and or high temperatures, drying, radiation, preservatives, and. Add aseptically 10 g of the food product after thorough mixing of the sample to 100 ml of selenite broth and add a further 10 g to 100 ml of tetrathionate broth. Selenite broth is used as an enrichment medium for the isolation of salmonella and shigella from stool, urine, water and food products. Subcultures should be made from the top of the broth after not more than 24 hours incubation as after this time there is.
A small amount of colored precipitate is not detrimental. Selenite f broth principle, uses, preparation and result interpretation. Sodium biselenite acts as the selective agent, while lactose is added as a fermentable carbohydrate to prevent a rise in ph value during incubation because any increase in ph will reduce the selective activity of selenite. Selenous acid, the conjugate acid, with the formula h 2 seo 3. Of these, 96 76% were isolated with selenite f broth and 118 94% were isolated with strontium selenite broth. Tryptone atau polypeptone 5 g lactose 4 g sodium acid selenite nahso4 4 g na2hpo4 10 g lcystine 0,01 g aquadest 1 liter panaskan sampai mendidih agar melarut. Selective enrichment of salmonella from foods format.
Selenite broth selenitef broth laboratoires humeau. Selenite broth selenitef broth is used as an enrichment medium for the isolation of salmonella from feces, urine, water, foods and other materials of sanitary importance. Selenite f broth is the medium used for the selective enrichment of salmonella spp from both clinical and food samples. Composition ingredients gramslitre ingredients gramslitre part a peptic digest of animal tissue 5. Before antibiotic therapy, strontium selenite broth alone yieldeds. Selenite broth was originated by leifson, while observing good recovery of salmonella spp. Composition ingredients gms litre part a tryptone 5.
Can we use selenite f broth media to revive organisms like pseudomonas aeruginosa, staphylococcus aureus, micrococcus spp. Ivesons revised formula personal communication as follows. Selenite broth was originated by leifson, while observing good recovery of. Handbook of media for clinical and public health microbiology. Selenite ion, anion is a selenium oxoanion with the chemical formula seo 3 2. Selenite f broth principle, uses, preparation and result. It is recommended by bis committee composition ingredients gms litre part a peptone 5. Intended use hardy diagnostics criterion selenite cystine broth is a selective enrichment for isolation of salmonella spp.
1627 1354 325 1027 204 1592 1227 453 950 487 1515 1306 1087 1648 904 864 1431 500 1032 1608 1028 266 1274 356 1355 187 592 1105 779 73 1376 1501 542 9 73 970 844 472 1454 794